what cell density to use for mcf-7 and mda-mb-231 mtt/mts
What cell density to use for MCF-7 and MDA-MB-231 MTT/MTS
I have read somewhere that some drugs work well in high and low cell density/proliferation rates and some drugs work only on rapidly dividing cells. What is the optimal cell density for MCF-7 and ...
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Differential Expression of HSP90β in MDA-MB-231 and MCF-7
The present study aimed to investigate two different breast cancer cell lines, MCF-7 and MDA-MB-231. MCF- 7 cells are estrogen receptor (ER)-positive cells that have been widely used to study estrogen-related pathways and the development of anti-hormonal therapies for breast cancer [17–19].
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The Impact of Soy Isoflavones on MCF-7 and MDA-MB-231
Exposing MCF-7 and MDA-MB-231 cells to high isoflavones doses (IC 20) hindered cell proliferation. In both cell lines, isoflavone exposure led to a deficient glucose uptake, depriving cells of their main energy source. The glucose influx was restricted more in MCF-7 cells than in MDA-MB-231 cells.
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Growth curve protocol for mda-mb-231 cell line?
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay. Can you help by adding ...
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I am trying to induce EMT in breast cancer cell lines (MCF
All Answers ( 13) MCF-7 line is better for basal level of Ecad. You can enhance the E-cad by lower % of FCS, which is good also to add any cytokines and work with these conditions. You can use EGF (or combinations) to induce EMT in breast cancer cell lines like MCF7.
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How to culture MCF-7 cell line? - ResearchGate
Please go to ATCC website, American Type Cell Culture collection, they will give you details of how to culture These breast cancer cell lines. Once you open the main page, type MCF-7 in the search mode. You can try follwoing remedies, one by one: 1. While sub-culture, you may follow following steps-a.
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How long does it take to culture MCF7 cells and subdivide
How long does it take to culture MCF7 cells and subdivide into 1:4 ratio? ... What cell density to use for MCF-7 and MDA-MB-231 MTT/MTS assay after treatment with common cancer chemo agents ...
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Is there any way to make MCF7 cells invade at the baseline
In our hands regular MCF-7 never invade, but there is at least two papers that can help you. They select the MCF-7 cells by prolonged mammosphere culture, and claim that the cells selected have metastatic potential. - Guttilla et al.
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Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line
Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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High Absorbance value (near 1.7) in my 24 hour MDA-MB-231
High Absorbance value (near 1.7) in my 24 hour MDA-MB-231 cells MTT assay? I did MTT assay to check the proliferation of MDA-MB-231 cells overtime (24,48 and 72 hours) in 96 well plate.
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What cell density to use for MCF-7 and MDA-MB-231 MTT/MTS
I have read somewhere that some drugs work well in high and low cell density/proliferation rates and some drugs work only on rapidly dividing cells. What is the optimal cell density for MCF-7 and
Send Inquiry
Differential Expression of HSP90β in MDA-MB-231 and MCF-7
The present study aimed to investigate two different breast cancer cell lines, MCF-7 and MDA-MB-231. MCF- 7 cells are estrogen receptor (ER)-positive cells that have been widely used to study estrogen-related pathways and the development of anti-hormonal therapies for breast cancer [17–19].
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The dose dependent in vitro responses of MCF-7 and MDA-MB
The MCF-7 and the MDA-MB-231 cells were treated with the extract at half IC 50 (0.5 IC 50) concentration for up to 48 h, then released by trypsinizing the cells and collected by centrifugation. The cells were washed once in cold PBS, and lysed cells in radioimmunoprecipitation assay buffer (Peirce Biotechnology, IL, USA).
Send InquiryGrowth curve protocol for mda-mb-231 cell line?
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay. Can you help by adding
Send Inquiry
I am trying to induce EMT in breast cancer cell lines (MCF
All Answers ( 13) MCF-7 line is better for basal level of Ecad. You can enhance the E-cad by lower % of FCS, which is good also to add any cytokines and work with these conditions. You can use EGF (or combinations) to induce EMT in breast cancer cell lines like MCF7.
Send Inquiry
Is there any way to make MCF7 cells invade at the baseline
In our hands regular MCF-7 never invade, but there is at least two papers that can help you. They select the MCF-7 cells by prolonged mammosphere culture, and claim that the cells selected have metastatic potential. - Guttilla et al.
Send Inquiry
How to culture MCF-7 cell line? - ResearchGate
Please go to ATCC website, American Type Cell Culture collection, they will give you details of how to culture These breast cancer cell lines. Once you open the main page, type MCF-7 in the search mode. You can try follwoing remedies, one by one: 1. While sub-culture, you may follow following steps-a.
Send Inquiry
How long does it take to culture MCF7 cells and subdivide
How long does it take to culture MCF7 cells and subdivide into 1:4 ratio? What cell density to use for MCF-7 and MDA-MB-231 MTT/MTS assay after treatment with common cancer chemo agents
Send Inquiry